R & F® Campylobacter jejuni/C. coli Chromogenic Plating 
Medium

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Selective/Differential System For Identifying Campylobacter jejuni/C. coli

Colony Appearance

Campylobacter jejuni/C. coli appear as dark salmon flat to convex colonies, 1.0-2.0 mm in diameter with and without a clear ring after 48 h at 41-42°C under microaerophilic conditions. C. lari appears as beige to off-white colonies 1.0-2.0 mm in diameter. Ninety five percent of the Gram (+)s, Gram (-)s and yeasts did not grow, 5% grew as pinpoint white colonies.

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Mechanism

Our medium contains a unique chromogenic substrate that detects the C-2 esterase found in the thermophilic C. jejuni and C. coli but absent in C. lari. Other Campylobacter species (that may possess the C-2 esterase enzyme) are eliminated from this medium by selective ingredients and/or incubation conditions. The chromogenic substrate used to detect the C-2 esterase is stable in the plating medium held at 2-8°C and produces a water insoluble chromogen (red color) in the presence of the enzyme under anaerobic conditions. Therefore, no need for blood, charcoal or other oxygen scavenging compounds. An opaque white background is utilized for better observing the dark salmon colored colonies of C. jejuni and C. coli from the background flora, especially C. lari after incubation.
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Industry Types

Poultry, meat, dairy products including fluid milk, and untreated drinking water

See Product Brochure, Use Protocol, SDS, Certificate of Analysis and Medium Preparation for more information about our medium.
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Advantages/Benefits

1. Our medium is the first chromogenic plating medium to specifically isolate C. jejuni and C. coli.


2. Our medium, via the chromogenic substrate, detects the C-2 esterase which is found in the thermophilic

C. jejuni and C. coli but not C. lari.


3. Blood and/or charcoal are not required to isolate C. jejuni and C. coli.


4. Highly selective/differential detection system with a selectivity and sensitivity for C. jejuni and C. coli of 98% and 100%, respectively, with no false negatives (>90 C. jejuni and C. coli strains tested).


5. Water insoluble chromogenic lattice prevents the colony color from diffusing into the agar making detection and enumeration much easier.


6. Plates may be examined after 24 hours for C. jejuni and C. coli.


7. Selectivity of medium and incubation conditions inhibits the growth of most enterics and other closely related Gram negative bacteria.


8. In competitors’ media, all bacterial colonies have the same colony color with no differentiating capabilities other than size and shape, resulting in more costly tests as well as labor costs.


9. The shelf life of prepared plates remains stable for at least 60 days, stored in the dark at 2-8°C. One bottle of plating medium powder and one box of supplements will make approximately 255 plates.


10. Tested by the FDA, USDA and state departments of health and agriculture with excellent results.

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Publications

1. Restaino, Lawrence. 2012. A novel chromogenic plating medium for the isolation of Campylobacter jejuniand Campylobacter coli. Poster No. 0101-000046, International Poultry Expo/International Feed Expo 2012.

2. Wang, Q. et al. FDA Campylobacter jejuni and coli Detection Method from Raw Silo Milk. Poster IAFP 2012.
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Ordering Information

M-0950

R & F® Campylobacter jejuni/C. coli Chromogenic Detection System (250 g Bottle of Powder & one Supplement Box – Makes 250-260 Plates)

M-0900

R & F® Campylobacter jejuni/C. coli chromogenic Plating Medium (250 g Bottle)

M-0910

R & F® Campylobacter jejuni/C. coli Supplement for Plating Medium (1 Box)

M-0960

R & F® Campylobacter jejuni/C. coli Chromogenic Prepared Plate (Minimum Order of 20 Plates – Shelf life is 60 Days)