R & F® Salmonella (Nontyphoidal) Chromogenic Plating Medium

R & F® Salmonella (Nontyphoidal) Chromogenic Plating Medium

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Selective/Differential Medium For Identifying Salmonella Excluding Salmonella Typhi/Paratyphi

Colony Appearance

Presumptive positive colonies of Salmonella appear as reddish-pink raised colonies 1.0-3.0 mm in diameter with colorless ring after 20-24 h at 35°C. Only Salmonella Bongori grew as dark-blue colony. One Escherichia coli strain grew as red-pink raised color colony 2.0 mm in diameter. All other enterics will either not grow or produce green, blue-green, purple, blue, tan or colorless colonies. The Gram (+)s, pseudomonads, Morganella morganii, Pantoea sp., Acinetobacter sp., Shigella sp., Salmonella Typhi and Salmonella Paratyphi are inhibited.

Mechanism

The Salmonella (Nontyphoidal) medium like the Salmonella (includes S. Typhi) medium use the carbohydrate 2-deoxy-D-ribose which is utilized almost exclusively by Salmonella strains and in the presence of a pH indicator produces pink to red colored colonies. A portion of other bacteria that may ferment this sugar includes some Citrobacter and E. coli strains. These bacterial strains are eliminated from looking like Salmonella colonies by splitting the chromogenic substrate X-β-D-galactopyranoside by using the enzyme β-galactosidase which is present in Citrobacter and E. coli but not Salmonella. Consequently, these strains of E. coli and Citrobacter will produce purple colonies. With increased antibiotics and additional selective ingredients, the Salmonella (Nontyphoidal) medium is better suited for testing the Salmonella pathogen in foods, whereas, S. Typhi/Paratyphi is not a major concern in foods.

Industry Types

Fish, meat, poultry, dairy, egg, produce, fruits, ready-to-eat foods and drinking water – includes most food industry types

See Product Brochure, Use Protocol, SDS, Certificate of Analysis and Medium Preparation for more information about our medium.

Advantages/Benefits

1. Our medium is unique in that only non-coliform enterics that grow on our medium and ferments the 2-deoxy-D-ribose sugar are Salmonella sp. (76 Salmonella stains tested -all 4 Salmonella subgroups fermented the 2-deoxy-D-ribose sugar).


2. Highly selective chromogenic medium that isolates Salmonella sp., excluding S. Typhi/Paratyphi, while differentiating from or inhibiting the growth of many similar bacteria which can interfere with Salmonellagrowth and identification in foods (meats and produce).


3. High specificity and sensitivity; differentiates Salmonella and Citrobacter while inhibiting other bacteria, such as pseudomonads, Morganella morganii, Pantoea sp. and Gram (+)s. Reduced growth (≤1.0 mm) occurs with generic E. coli, Citrobacter sp., Klebsiella sp., Providencia sp. and Hafnia alvei.


4. Most competitors’ chromogenic media use the C-8 esterase, detected by the caprylate chromogenic substrate, which is sensitive for Salmonella sp. However, the C-8 esterase activity is also positive for some non-enterics (i.e. Pseudomonas, Acinetobacter sp. and Aeromonas sp.) and enterics (i.e. Enterobacter sp., Klebsiella pneumoniae, Hafnia alvei and Citrobacter freundii) which eventually will interfere with Salmonelladetection–false positives.


5. The cost of the C-8 esterase detection chromogenic substrate is more expensive as compared to our Salmonella medium.


6. Our medium results in a simple and inexpensive agar for detection and confirmation of Salmonella.


7. The shelf life of prepared plates remains stable for at least 60 days, stored in the dark at 2-8°C. One bottle of plating medium powder and one box of supplements will make approximately 525 plates.


8. FDA tested and used by several state departments of health and agriculture, food manufacturing and processing facilities and private testing labs.

Ordering Information

M-1350R & F® Salmonella (Nontyphoidal) Chromogenic Detection System (400 g Bottle of Powder & one Supplement Box – Makes – 500-550 Plates)

M-1300

R & F® Salmonella (Nontyphoidal) Chromogenic Plating Medium (400 g Bottle)

M-0610

R & F® Salmonella Supplement for Plating Medium (1 Box)

M-1360

R & F® Salmonella (Nontyphoidal) Chromogenic Prepared Plate (Minimum Order of 20 Plates – Shelf life is 60 Days)

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