Highly Selective Enrichment Broth That Promotes The Growth Of Arcobacter butzleri, A. cryaerophilus, and A. skirrowii.

Broth Function

The purpose of an enrichment broth is to amplify very low levels of a specific bacterium within 24 to 48 hours so it can be more readily identified and isolated by a subsequent methodology. In situations where cells of the targeted bacterium are very low and injury is high, such cells must undergo repair without deleterious effects. The broth must also contain selective agents to suppress background growth which can interfere with the isolation of the target bacterium. The development of our enrichment broth was specifically formulated for the rapid growth and isolation of Arcobacter butzleri, A. cryaerophilus, and A. skirrowii.

See Product Brochure, Use Protocol, SDS, Certificate of Analysis and Medium Preparation for more information about our medium.

1. One-hundred percent inclusivity for the growth of A. butzleri, A. cryaerophilus, and A. skirrowii strains tested after an incubation period of 48 hours at 30°C under aerobic conditions.

2. Selectivity includes the elimination of most pseudomonad-type bacteria as well as Gram (+) bacteria and yeasts.

3. Ease of preparation with forty-five day shelf stability of broth stored at 2-8°C in the dark.

4. One bottle of broth powder and one box of supplements will make approximately 11.5 liters of enrichment broth.

1. Nguyen, P.T., K. Tuz, O. Juárez, and L. Restaino. 2023. Comparison of Two Culture-Based Detection Systems for the Isolation of Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii in Raw Ground Poultry. J. Food Prot. 86(3): 100057.

2. Nguyen, P.T., K. Tuz, O. Juárez, and L. Restaino. 2022. NRJ Media as the Gold-Standard Arcobacter-specific Detection System: Applications in Poultry Testing. Front. Microbiol. 13.903079.

3. Nguyen, P.T., O. Juárez, and L. Restaino. 2021. A New Method for Detection of Arcobacter butzleri, A. cryaerophilus, and A. skirrowii using a Novel Chromogenic Agar. J. Food Prot. 84(1): 160-168