R & F® Salmonella (includes S. Typhi) Chromogenic Plating Medium

R & F® Salmonella (includes S. Typhi) Chromogenic Plating Medium

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Colony Appearance

Presumptive positive colonies of Salmonella appear as reddish-pink raised colonies 1.0-3.0 mm in diameter with colorless ring after 20-24 h at 35°C. Only Salmonella Bongori grew as dark-blue colony. One Escherichia coli strain grew as red-pink raised color colony 2.0 mm in diameter. All other enterics will either not grow or produce green, blue-green, purple, blue, tan or colorless colonies. The Gram (+)s and pseudomonads are inhibited.

Mechanism

Both of our Salmonella media (includes S. Typhi medium and S. Nontyphoidal medium) use the carbohydrate 2-deoxy-D-ribose which is utilized almost exclusively by Salmonella strains. Coupled with the pH indicator, Salmonella produces pink to red colored colonies. A portion of other bacteria that may ferment this sugar includes Citrobacter (about 50% of the strains) and E. coli (about 40% of the strains). These bacterial strains are eliminated from looking like Salmonella colonies by splitting the chromogenic substrate X-β-D-galactopyranoside using the enzyme β-galactosidase which is present in Citrobacter and E. coli but notSalmonella. Consequently, these strains of E. coli and Citrobacter will produce purple colonies. The Salmonella (includes S. Typhi) medium is more suited for clinical and water samples because of the lower concentrations of inhibitors/antibiotics as compared to the Salmonella (Nontyphoidal) medium.

Industry Types

Clinical, drinking and environmental water and soils

See Product Brochure, Use Protocol, SDS, Certificate of Analysis and Medium Preparation for more information about our medium.

Advantages/Benefits

1. Our medium is unique in that only non-coliform enterics that grow on our medium and ferments the 2-deoxy-D-ribose sugar are Salmonella sp. (80 Salmonella strains tested -all 4 subgroups of Salmonellaincluding Typhi/Paratyphi fermented the 2-deoxy-D-ribose sugar).


2. E. coli and Citrobacter sp. strains that ferment and do not ferment 2-deoxy-D-ribose are differentiated from Salmonella by being positive for β-galactosidase, producing purple and blue-green colonies, respectively.


3. High sensitivity and specificity and has been used in clinical labs for testing.


4. Most competitors’ chromogenic media use the C-8 esterase, detected by the caprylate chromogenic substrate, which is sensitive for Salmonella sp. However, the C-8 esterase activity is also positive for some non-enterics (i.e. Pseudomonas, Acinetobacter sp. and Aeromonas sp.) and enterics (i.e. Enterobacter sp., Klebsiella pneumoniae, Hafnia alvei and Citrobacter freundii) which eventually will interfere with Salmonelladetection–false positives.


5. The cost of the C-8 esterase detection chromogenic substrate is more expensive as compared to our Salmonella medium.


6. Our medium results in a simple and inexpensive agar for detection and confirmation of Salmonella.


7. The shelf life of prepared plates remains stable for at least 60 days, stored in the dark at 2-8°C. One bottle of plating medium powder and one box of supplements will make approximately 525 plates.

Publications

1. Reissbrodt, R., B. Burghardt, and H. Tschäpe. 2001. Evaluation of four new chromogenic Salmonella plating media. Robert Koch-Institut (Poster Session).

Ordering Information

M-0650R & F® Salmonella (S. Typhi) Chromogenic Detection System (400 g Bottle of Powder & one Supplement Box – Makes – 500-550 Plates)

M-0600

R & F® Salmonella (S. Typhi) Chromogenic Plating Medium (400 g Bottle)

M-0610

 R & F® Salmonella Supplement for Plating Medium (1 Box)

M-0660

R & F® Salmonella (S. Typhi) Chromogenic Prepared Plate (Minimum Order of 20 Plates – Shelf life is 60 Days)

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