R & F® Yersinia pestis Chromogenic Plating Medium
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Colony Appearance
Presumptive positive colonies of Yersinia pestis appear as blue-black raised colonies 0.5-2.0 mm in diameter with or without colorless halos after 48-72 h at 26-30°C. Y. enterocolitica, Y. pseudotuberculosis, Y. kristensenii, Y. intermedia and other Yersinia species will grow as green to yellow colonies 1.0-2.0 mm in diameter. Other Gram (-)s either are inhibited or grow as green, blue-green, yellow or white colonies.
Gram (+)s are inhibited.
Mechanism
The Yersinia pestis medium uses the chromogenic substrate X-β-D-glucopyranoside to detect the enzyme β-glucosidase which is found in all Y. pestis strains. In addition, this medium contains sucrose, L-rhamnose, cellobiose, and lactose which Y. pestis cannot ferment. Therefore, the Y. pestis produces a blue-black colony in the presence of the pH indicator brom cresol purple, whereas other Yersina species which ferment one of the sugars produce green to yellow colored colonies.
Industry Types
Bioterrorism (environmental, drinking water and food)
See Product Brochure, Use Protocol, SDS, Certificate of Analysis and Medium Preparation for more information about our medium.
Advantages/Benefits
1. The medium has been collaboratively developed and evaluated by the FDA and is the medium of choice to enhance Yersinia pestis identification in dealing with the potential bioterrorism threat.
2. The only chromogenic product on the market; uses the X-β-D-glucopyranoside to detect β-glucosidase in Yersinia pestis – reduces false positives/false negatives.
3. Single-step identification of Yersinia pestis, does not require the use of multiple media for isolation of presumptive Yersinia pestis colonies.
4. Readily differentiates Yersinia pestis from related Y. enterocolitica, Y. pseudotuberculosis, Y. kristensenii, Y. intermedia and other Yersinia species.
5. Efficient recovery from foods, environmental sources, and clinical samples.
6. The shelf life of prepared plates remains stable for at least 14 days, stored in the dark at 2-8°C. One bottle of plating medium powder and one box of supplements will make approximately 325 plates.
7. Listed in the FERN (Food Emergency & Response Network) and has been tested by the FDA, USDA, CDC, state departments of health and agriculture and the military.
Ordering Information
M-1050
R & F® Yersinia pestis Chromogenic Detection System (500 g Bottle of Powder & one Supplement Box – Makes – 320-330 Plates)
M-1000
R & F® Yersinia pestis Chromogenic Plating Medium (500 g Bottle)
M-1010
R & F® Yersinia pestis Supplement for Plating Medium (1 Box)
M-1060
R & F® Yersinia pestis Chromogenic Prepared Plate (Minimum Order of 20 Plates – Shelf life is 14 Days)
